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murine ifnλ2  (PeproTech)


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    PeproTech murine ifnλ2
    Increased activation and transferrin receptor expression after IFNα stimulation of NK cells. Expanded NK cells were stimulated with IFNα11 (500 U/ml), IFNβ (500 U/ml), <t>IFNλ2</t> (100 ng/ml), IL-2/12 (20 ng/ml, 10 ng/ml) for 18 hours or left unstimulated (unstim.). NK cells (viable, CD3 - , NK1.1 + ) were analyzed for activation (CD69) (A) . Representative dot plots and bar graphs of CD71 + NK cells and transferrin receptor (CD71) expression on CD71 + NK cells are shown in (B–D) . Statistically significant differences between the groups were analyzed with an Ordinary one-way ANOVA. All data are presented as mean values ± SEM. In (E, F) , correlation of CD71 (MFI, %) and CD69 (%) is shown. Splenocytes were stimulated or left untreated overnight and the uptake of transferrin was measured in NK cells by flow cytometry (G) . Correlation of transferrin uptake and activation is shown in (H) . Five mice from 3 independent experiments were used and analyzed by an ordinary one-way ANOVA. Black circles represent unstimulated, open circles IFNα-, grey IFNβ-, dark grey IFNλ- and bright grey IL-2/-12-stimulated NK cells. In (I) , the NK cell proliferation was analyzed by using KI-67. For (A, C–F) five mice per group collected from five independent experiments were used (n=5).
    Murine Ifnλ2, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+ifn%CE%BB2/ifn+%CE%BB1/pmc11772171-84-19-23
    Average 90 stars, based on 1 article reviews
    murine ifnλ2 - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Iron improves the antiviral activity of NK cells"

    Article Title: Iron improves the antiviral activity of NK cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2024.1526197

    Increased activation and transferrin receptor expression after IFNα stimulation of NK cells. Expanded NK cells were stimulated with IFNα11 (500 U/ml), IFNβ (500 U/ml), IFNλ2 (100 ng/ml), IL-2/12 (20 ng/ml, 10 ng/ml) for 18 hours or left unstimulated (unstim.). NK cells (viable, CD3 - , NK1.1 + ) were analyzed for activation (CD69) (A) . Representative dot plots and bar graphs of CD71 + NK cells and transferrin receptor (CD71) expression on CD71 + NK cells are shown in (B–D) . Statistically significant differences between the groups were analyzed with an Ordinary one-way ANOVA. All data are presented as mean values ± SEM. In (E, F) , correlation of CD71 (MFI, %) and CD69 (%) is shown. Splenocytes were stimulated or left untreated overnight and the uptake of transferrin was measured in NK cells by flow cytometry (G) . Correlation of transferrin uptake and activation is shown in (H) . Five mice from 3 independent experiments were used and analyzed by an ordinary one-way ANOVA. Black circles represent unstimulated, open circles IFNα-, grey IFNβ-, dark grey IFNλ- and bright grey IL-2/-12-stimulated NK cells. In (I) , the NK cell proliferation was analyzed by using KI-67. For (A, C–F) five mice per group collected from five independent experiments were used (n=5).
    Figure Legend Snippet: Increased activation and transferrin receptor expression after IFNα stimulation of NK cells. Expanded NK cells were stimulated with IFNα11 (500 U/ml), IFNβ (500 U/ml), IFNλ2 (100 ng/ml), IL-2/12 (20 ng/ml, 10 ng/ml) for 18 hours or left unstimulated (unstim.). NK cells (viable, CD3 - , NK1.1 + ) were analyzed for activation (CD69) (A) . Representative dot plots and bar graphs of CD71 + NK cells and transferrin receptor (CD71) expression on CD71 + NK cells are shown in (B–D) . Statistically significant differences between the groups were analyzed with an Ordinary one-way ANOVA. All data are presented as mean values ± SEM. In (E, F) , correlation of CD71 (MFI, %) and CD69 (%) is shown. Splenocytes were stimulated or left untreated overnight and the uptake of transferrin was measured in NK cells by flow cytometry (G) . Correlation of transferrin uptake and activation is shown in (H) . Five mice from 3 independent experiments were used and analyzed by an ordinary one-way ANOVA. Black circles represent unstimulated, open circles IFNα-, grey IFNβ-, dark grey IFNλ- and bright grey IL-2/-12-stimulated NK cells. In (I) , the NK cell proliferation was analyzed by using KI-67. For (A, C–F) five mice per group collected from five independent experiments were used (n=5).

    Techniques Used: Activation Assay, Expressing, Flow Cytometry

    Related Articles

    Activation Assay:

    Article Title: Iron improves the antiviral activity of NK cells
    Article Snippet: were isolated from naive C57BL/6 mice and cultured for 6 days in 12.5 ng/ml IL-15 (BioLegend). .. On day 6, IL-2 (20 ng/ml), IL-12 (10 ng/ml), murine IFNα11 (500 U/ml) , murine IFNβ (500 U/ml) or murine IFNλ2 (100 ng/ml, Peprotech) were added for 18 hours, if indicated. .. Cells were incubated in a humidified atmosphere with 5% CO 2 at 37°C.

    Expressing:

    Article Title: Iron improves the antiviral activity of NK cells
    Article Snippet: were isolated from naive C57BL/6 mice and cultured for 6 days in 12.5 ng/ml IL-15 (BioLegend). .. On day 6, IL-2 (20 ng/ml), IL-12 (10 ng/ml), murine IFNα11 (500 U/ml) , murine IFNβ (500 U/ml) or murine IFNλ2 (100 ng/ml, Peprotech) were added for 18 hours, if indicated. .. Cells were incubated in a humidified atmosphere with 5% CO 2 at 37°C.

    Flow Cytometry:

    Article Title: Iron improves the antiviral activity of NK cells
    Article Snippet: were isolated from naive C57BL/6 mice and cultured for 6 days in 12.5 ng/ml IL-15 (BioLegend). .. On day 6, IL-2 (20 ng/ml), IL-12 (10 ng/ml), murine IFNα11 (500 U/ml) , murine IFNβ (500 U/ml) or murine IFNλ2 (100 ng/ml, Peprotech) were added for 18 hours, if indicated. .. Cells were incubated in a humidified atmosphere with 5% CO 2 at 37°C.



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    murine ifnλ2  (PeproTech)
    90
    PeproTech murine ifnλ2
    Increased activation and transferrin receptor expression after IFNα stimulation of NK cells. Expanded NK cells were stimulated with IFNα11 (500 U/ml), IFNβ (500 U/ml), <t>IFNλ2</t> (100 ng/ml), IL-2/12 (20 ng/ml, 10 ng/ml) for 18 hours or left unstimulated (unstim.). NK cells (viable, CD3 - , NK1.1 + ) were analyzed for activation (CD69) (A) . Representative dot plots and bar graphs of CD71 + NK cells and transferrin receptor (CD71) expression on CD71 + NK cells are shown in (B–D) . Statistically significant differences between the groups were analyzed with an Ordinary one-way ANOVA. All data are presented as mean values ± SEM. In (E, F) , correlation of CD71 (MFI, %) and CD69 (%) is shown. Splenocytes were stimulated or left untreated overnight and the uptake of transferrin was measured in NK cells by flow cytometry (G) . Correlation of transferrin uptake and activation is shown in (H) . Five mice from 3 independent experiments were used and analyzed by an ordinary one-way ANOVA. Black circles represent unstimulated, open circles IFNα-, grey IFNβ-, dark grey IFNλ- and bright grey IL-2/-12-stimulated NK cells. In (I) , the NK cell proliferation was analyzed by using KI-67. For (A, C–F) five mice per group collected from five independent experiments were used (n=5).
    Murine Ifnλ2, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+ifn%CE%BB2/ifn+%CE%BB1/pmc11772171-84-19-23
    Average 90 stars, based on 1 article reviews
    murine ifnλ2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Increased activation and transferrin receptor expression after IFNα stimulation of NK cells. Expanded NK cells were stimulated with IFNα11 (500 U/ml), IFNβ (500 U/ml), IFNλ2 (100 ng/ml), IL-2/12 (20 ng/ml, 10 ng/ml) for 18 hours or left unstimulated (unstim.). NK cells (viable, CD3 - , NK1.1 + ) were analyzed for activation (CD69) (A) . Representative dot plots and bar graphs of CD71 + NK cells and transferrin receptor (CD71) expression on CD71 + NK cells are shown in (B–D) . Statistically significant differences between the groups were analyzed with an Ordinary one-way ANOVA. All data are presented as mean values ± SEM. In (E, F) , correlation of CD71 (MFI, %) and CD69 (%) is shown. Splenocytes were stimulated or left untreated overnight and the uptake of transferrin was measured in NK cells by flow cytometry (G) . Correlation of transferrin uptake and activation is shown in (H) . Five mice from 3 independent experiments were used and analyzed by an ordinary one-way ANOVA. Black circles represent unstimulated, open circles IFNα-, grey IFNβ-, dark grey IFNλ- and bright grey IL-2/-12-stimulated NK cells. In (I) , the NK cell proliferation was analyzed by using KI-67. For (A, C–F) five mice per group collected from five independent experiments were used (n=5).

    Journal: Frontiers in Immunology

    Article Title: Iron improves the antiviral activity of NK cells

    doi: 10.3389/fimmu.2024.1526197

    Figure Lengend Snippet: Increased activation and transferrin receptor expression after IFNα stimulation of NK cells. Expanded NK cells were stimulated with IFNα11 (500 U/ml), IFNβ (500 U/ml), IFNλ2 (100 ng/ml), IL-2/12 (20 ng/ml, 10 ng/ml) for 18 hours or left unstimulated (unstim.). NK cells (viable, CD3 - , NK1.1 + ) were analyzed for activation (CD69) (A) . Representative dot plots and bar graphs of CD71 + NK cells and transferrin receptor (CD71) expression on CD71 + NK cells are shown in (B–D) . Statistically significant differences between the groups were analyzed with an Ordinary one-way ANOVA. All data are presented as mean values ± SEM. In (E, F) , correlation of CD71 (MFI, %) and CD69 (%) is shown. Splenocytes were stimulated or left untreated overnight and the uptake of transferrin was measured in NK cells by flow cytometry (G) . Correlation of transferrin uptake and activation is shown in (H) . Five mice from 3 independent experiments were used and analyzed by an ordinary one-way ANOVA. Black circles represent unstimulated, open circles IFNα-, grey IFNβ-, dark grey IFNλ- and bright grey IL-2/-12-stimulated NK cells. In (I) , the NK cell proliferation was analyzed by using KI-67. For (A, C–F) five mice per group collected from five independent experiments were used (n=5).

    Article Snippet: On day 6, IL-2 (20 ng/ml), IL-12 (10 ng/ml), murine IFNα11 (500 U/ml) , murine IFNβ (500 U/ml) or murine IFNλ2 (100 ng/ml, Peprotech) were added for 18 hours, if indicated.

    Techniques: Activation Assay, Expressing, Flow Cytometry